Cypress CSC-5300 Manual de usuario descargar pdf (Pagina 57)

50 Chapter 5: Software Description

Calorimetry Sciences Corp.

CSC 5300 N-ITC III 51

User’s Manual

Modeling

Modeling Overview

Each model contains a set of variable parameters. Curve tting produces optimum values

for these parameters. These values will best represent the given input data set.

In addition to user-dened binding models, BindWorks supports three intrinsic binding

models:

1. Independent Set of Multiple Binding Sites.

2. Two Sets of Multiple Binding Sites.

3. Cooperative Model.

Intrinsic models are “hard-wired” in BindWorks and cannot be changed or replaced.

Independent Set of Multiple Binding Sites

This is the most common model for a binding experiment. The analytical solution for the

total heat is determined by the formula:

Where the variable parameters for the model are:

∆H - is the enthalpy of binding

K - is the binding constant

n - is the number of binding sites

Other variables include:

Equation 5-3













⋅

⋅⋅+⋅−⋅⋅+−⋅⋅+

+⋅∆⋅=

LKKLKnMKnM

LHVQ

][4)][][1(][1

][

1 2 ... 52 53 54 55 56 57 58 59 60 61 62 ... 75 76

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Cypress CSC-5300 Manual de usuario Pagina 57