Cypress CSC-5300 Manual de usuario descargar pdf (Pagina 27)

20 Chapter 3: Theory of Operation

Calorimetry Sciences Corp.

CSC 5300 N-ITC III 21

User’s Manual

Freire, E., Mayorga, O. L. & Straume, M. (1990). Isothermal Titration

Calorimetry. Anal. Chem. 62, 950A-959A.

Wiseman, T., Williston, S., Brandts, J. F. & Lin, L.-N. (1989). Rapid

Measurement of Binding Constants and Heats of Binding Using a New

Titration Calorimeter. Anal. Biochem. 179, 131-137.

Wyman, J. & Gill, S. J. (1990). Binding and Linkage: The Functional

Chemistry of Biological Macromolecules (University Science Books, Mill

Valley).

Fitting of Calorimetric Data

Values for K and ∆H are obtained by tting the integrated heats from a titration

experiment to Equation 3-1 or Equation 3-2. Use of Equation 3-2 is simpler and faster

as it requires only one calculation of the excess enthalpy for each injection whereas

Equation 3-1 requires two. In general, use of Equation 3-2 presents no problem, however

by using Equation 3-2, the error from all the previous injections is propagated into

each value of the cumulative heat. Better statistics are therefore obtained by tting the

individual heats according to Equation 3-1.

1 2 ... 22 23 24 25 26 27 28 29 30 31 32 ... 75 76

Sin comentarios

Cypress CSC-5300 Manual de usuario Pagina 27